Table 1 Primers and PCR conditions used in DNA sequencing
From: Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales
Gene | Primers | Oligonucleotides (5′-3′) | References |
|---|---|---|---|
Large subunit ribosomal DNA (LSU) | NL1 | GCATATCAATAAGCGGAGGAAAAG | |
LR5 | TCCTGAGGGAAACTTCG | ||
Internal Transcribed Spacer rDNA (ITS) | ITS1 | TCCGTAGGTGAACCTGCGG | (White et al. 1990) |
ITS4 | TCCTCCGCTTATTGATATGC | ||
β-tubulin (BT2) | Bt-2a | GGTAACCAAATCGGTGCTGCTTTC | (Glass & Donaldson 1995) |
| Â | Bt-2b | ACCCTCAGTGTAGTGACCCTTGGC | |
Translation Elongation factor 1-α (TEF1) | EF1-728F | CATCGAGAAGTTCGAGAAGG | (Carbone & Kohn 1999) |
EF1-986R | TACTTGAAGGAACCCTTACC |