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Fig. 7 | IMA Fungus

Fig. 7

From: Comprehensive analysis of full genome sequence and Bd-milRNA/target mRNAs to discover the mechanism of hypovirulence in Botryosphaeria dothidea strains on pear infection with BdCV1 and BdPV1

Fig. 7

Sequence and expression analyses of the RNA-silencing components of Botryosphaeria dothidea. a Predicted domain structure of dicer (DCL), RNA-dependent RNA polymerases (RdRps) and argonaute (Ago) proteins in B. dothidea LW-Hubei strain. Lines represent the full length of proteins. Boxes represent the identified domains and are labeled with different colors. b Phylogenetic analyses of dicer (left), RdRps (right), and Ago (middle) protein sequences from B. dothidea and homologous proteins from representative members of other fungi. Counterparts from Arabidopsis thaliana served as the outgroup member. Rooted maximum likelihood neighbor-joining trees with 1000 bootstrap replicates were constructed by alignment of full-length protein sequences. The accession numbers for dicer, RdRps, and AGO protein sequences from representative members used in the alignment are listed in Additional file 30: Table S14. c Analysis of BdDicer, BdRdRp and BdAgo gene expression in mycovirus-infected B. dothidea LW-Hubei strains. Values were normalized to the mean actin gene (GME8592_g) expression

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