Table 4 The patterns of differentially expressed Bd-milRNAs and targeted gene mRNAs analyzed in mycelia from Botryosphaeria dothidea strains cultured for 5 d following infection with BdCV1 and BdPV1 obtained by sRNA sequencing and RT-qPCR analysis, respectively
ID BdmiRNA | Log2FC | ID target gene | RT- qPCR | Function annotation | ||||
|---|---|---|---|---|---|---|---|---|
LW-CP/Mock | LW-C/Mock | LW-P/Mock | LW-CP/Mock | LW-C/Mock | LW-P/Mock | |||
Bd-milR12 | / | 10.22 | / | GME3643_g | 1.52 | 1.13 | 2.54 | Putative calcium-transporting P-type ATPase |
GME1913_g | 1.88 | 1.69 | 1.52 | Hypothetical protein MPH_03910 | ||||
Bd-milR33 | 8.55 | / | / | GME4906_g | 1.79 | 1.00 | 1.12 | Peptidase M43 pregnancy-associated plasma-A |
Bd-milR60 | −7.01 | −7.01 | − 7.01 | GME2439_g | 0.44 | 0.76 | 2.04 | Putative polyketide synthase protein |
GME5002_g | 1.91 | 2.38 | 1.80 | Hypothetical protein MPH_01630 | ||||
GME11323_g | 0.04 | 0.35 | 0.03 | Hypothetical protein UCDDS831_g08653 | ||||
Bd-milR1147.2 | / | 15.973 | / | GME2445_g | / | 2.76 | 1.40 | Multicopper oxidase type 1 |
GME4334_g | 1.11 | 0.93 | 1.50 | Putative ABC transporter protein | ||||
Bd-miR2094-5p | −3.561 | −15.79 | −3.259 | GME4116_g | 6.16 | 3.15 | 0.68 | Zinc finger C2H2-type protein |
Bd-milR46 | −7.37 | −7.37 | / | GME4518_g | 2.36 | 3.87 | 0.62 | Glucose-6-phosphate dehydrogenase |
Bd-milR6 | / | 6.66 | 6.78 | GME2361_g | 1.87 | 1.75 | 1.21 | Mannose-6-phosphate receptor binding protein |
GME985_g | 0.64 | 1.01 | 0.28 | Hypothetical protein MPH_12204 | ||||
Bd-milR65 | −7.72 | −7.72 | −7.72 | GME11656_g | 3.95 | 5.32 | 0.97 | MFS sugar transporter |
Bd-milR172c-3p | 2.94 | −14.26 | 2.68 | GME3697_g | 1.20 | 1.55 | 1.05 | Putative WD repeat domain 5b |
Bd-milR8635 | −2.82 | −5.34 | / | GME1751_g | 2.41 | 1.50 | 2.51 | Urea carboxylase |
GME4636_g | 1.16 | 0.86 | 0.45 | Putative ABC multidrug transporter mdr1 protein | ||||
Bd-milR5636 | / | 14.92 | / | GME10476_g | 1.89 | 1.24 | 2.81 | Cyclin-dependent protein kinase complex component |
GME1284_g | 1.44 | 0.28 | 1.41 | Hypothetical protein MPH_03537 | ||||
KF688736 | 8133.09 | 9243.67 | – | Putative RNA-dependent RNA polymerase (RdRp) gene | ||||
Bd-milR5568 | / | 14.12 | / | Unigene3269 | 128,971.41 | 11,426.09 | 47.78 | Hypothetical protein PGUG_01202 |
KF688737 | 2053.45 | 1537.81 | – | Putative coat protein (cp) gene | ||||