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Table 4 The patterns of differentially expressed Bd-milRNAs and targeted gene mRNAs analyzed in mycelia from Botryosphaeria dothidea strains cultured for 5 d following infection with BdCV1 and BdPV1 obtained by sRNA sequencing and RT-qPCR analysis, respectively

From: Comprehensive analysis of full genome sequence and Bd-milRNA/target mRNAs to discover the mechanism of hypovirulence in Botryosphaeria dothidea strains on pear infection with BdCV1 and BdPV1

ID BdmiRNALog2FCID target geneRT- qPCRFunction annotation
LW-CP/MockLW-C/MockLW-P/MockLW-CP/MockLW-C/MockLW-P/Mock
Bd-milR12/10.22/GME3643_g1.521.132.54Putative calcium-transporting P-type ATPase
    GME1913_g1.881.691.52Hypothetical protein MPH_03910
Bd-milR338.55//GME4906_g1.791.001.12Peptidase M43 pregnancy-associated plasma-A
Bd-milR60−7.01−7.01− 7.01GME2439_g0.440.762.04Putative polyketide synthase protein
   GME5002_g1.912.381.80Hypothetical protein MPH_01630
   GME11323_g0.040.350.03Hypothetical protein UCDDS831_g08653
Bd-milR1147.2/15.973/GME2445_g/2.761.40Multicopper oxidase type 1
   GME4334_g1.110.931.50Putative ABC transporter protein
Bd-miR2094-5p−3.561−15.79−3.259GME4116_g6.163.150.68Zinc finger C2H2-type protein
Bd-milR46−7.37−7.37/GME4518_g2.363.870.62Glucose-6-phosphate dehydrogenase
Bd-milR6/6.666.78GME2361_g1.871.751.21Mannose-6-phosphate receptor binding protein
   GME985_g0.641.010.28Hypothetical protein MPH_12204
Bd-milR65−7.72−7.72−7.72GME11656_g3.955.320.97MFS sugar transporter
Bd-milR172c-3p2.94−14.262.68GME3697_g1.201.551.05Putative WD repeat domain 5b
Bd-milR8635−2.82−5.34/GME1751_g2.411.502.51Urea carboxylase
   GME4636_g1.160.860.45Putative ABC multidrug transporter mdr1 protein
Bd-milR5636/14.92/GME10476_g1.891.242.81Cyclin-dependent protein kinase complex component
   GME1284_g1.440.281.41Hypothetical protein MPH_03537
   KF6887368133.099243.67Putative RNA-dependent RNA polymerase (RdRp) gene
Bd-milR5568/14.12/Unigene3269128,971.4111,426.0947.78Hypothetical protein PGUG_01202
   KF6887372053.451537.81Putative coat protein (cp) gene
  1. Note: “/” indicates no differential expression for Bd-milRNA in library; “-” indicates no significant data