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Fig. 3 | IMA Fungus

Fig. 3

From: Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses

Fig. 3

Production, purification and biochemical parameters of recombinant MCS. a -SDS-PAGE of different purification stages of MCS; Lane M – molecular weight marker, lane 1 whole bacterial cell lysate, lane 2 – flow-through from the Ni-resin, lane 3 – wash fraction through the resin, lanes 4–7 fractions eluted with 200 mM imidazole, lane 8 – concentrated pool of the MCS elution fraction (5 μg). b – Biochemical parameters of recombinant MCS

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