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Table 2 Methylcitrate synthase activity in cell extracts of yeast and mycelial forms of P. lutzii incubated in glucose, glucose with propionate or only propionate

From: Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses

 

Condition

MCS (SD)

MCS (SD) Dephosphorylated

Yeast

Glucose

0.510 (±0.005)

0.224 (±0.002)

Glucose/Propionate

0.708 (±0.005)

0.307 (±0.004)

Propionate

0.952 (±0.009)

0.391 (±0.008)

Mycelium

Glucose

2.421 (±0.068)

–

Glucose/Propionate

1.711 (±0.047)

–

Propionate

1.961 (±0.081)

–

  1. The data are defined as U × mg protein− 1. One unit of enzyme activity was defined as the amount of enzyme producing 1 μmol min− 1 of CoASH under the assay conditions. SD standard deviation; MCS methylcitrate synthase activity
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IMA Fungus

ISSN: 2210-6359

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