Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

IMA Fungus

Table 1 DNA Barcoding markers proposed for fungi, their recommended nomenclature and selected examples (see also Stielow et al. 2015; Xu 2016)

DNA barcoding marker	Acronym	Examples	References
Internal transcribed spacer	ITS	universal, Agaricus, Auricularia, Cora, Fomitopsis, Rhizoplaca, Sticta	Schoch et al. 2012; Leavitt et al. 2013; Lücking et al. 2014, 2017; Moncada et al. 2014, 2020; Irinyi et al. 2016; Badotti et al. 2017
Intergenic spacer	IGS	Schizophyllum	James et al. 2001
β-tubulin II	TUB2	Amanita, Aspergillus, Pseudopestalotiopsis	Geml et al. 2006; Geiser et al. 2007; Maharachchikumbura et al. 2012, 2014; Fidler et al. 2017
DNA-directed RNA polymerase II subunit A	RPB1	Inocybe	Matheny 2005
DNA-directed RNA polymerase II subunit B	RPB2	universal, Sordariomycetes, Cladonia, Inocybe	Matheny 2005; Pino-Bodas et al. 2013; Větrovský et al. 2016; Luo et al. 2019
Translation elongation factor 1 alpha	TEF1	universal, Sordariomycetes, Cantharellus, Fusarium, Trichoderma, Mycetozoa	Buyck and Hofstetter 2011; O'Donnell et al. 2015; Stielow et al. 2015; Schnittler et al. 2017; Luo et al. 2019
hypothetical protein	LNS2	Pucciniomycota	Stielow et al. 2015
Phosphoglycerate kinase	PGK	Fusarium, Penicillium	Al-Hatmi et al. 2016; Stielow et al. 2015
DNA topoisomerase I	TOP1	Pucciniomycota, Fusarium, Penicillium	Al-Hatmi et al. 2016; Stielow et al. 2015
Cytochrome c oxidase subunit I	COX1	Cladonia, Oomycota, Mycetozoa	Pino-Bodas et al. 2013; Choi et al. 2015; Schnittler et al. 2017
Cytochrome c oxidase subunit II	COX2	Oomycota	Choi et al. 2015

ISSN: 2210-6359