Fig. 2
From: Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis
Expression of the recombinant protein SidA and immunoblotting analysis. a SDS-PAGE of SidA expression by E. coli Rosetta (DE3). 79.5 kDa corresponds to 53.5 kDa of SidA fused to 26 kDa of GST-tag. b Membranes containing extracts of cytoplasmic proteins of P. brasiliensis cultivated in FOB or BPS for 24 h were incubated with pre-immune sera and anti-PbSidA polyclonal antibodies. The native SidA protein presents a molecular mass of 53.5 kDa (black arrow). c Membranes with the same extracts described above were incubated with anti-Pbenolase polyclonal antibodies. The pixel intensity of the immunoblotting bands was analyzed using the ImageJ 1.51 software and expressed as arbitrary units. The Student’s t test was used for statistical analysis. Error bars represent standard deviation of the experimental triplicate* p ≤ 0.05. MW: molecular weight marker. P0: E. coli before IPTG induction. IND: E. coli after IPTG induction (1 mM IPTG for 2 h)