Fig. 5

Production of siderophores in sidA silenced strains. a Wild type (WT), empty vector (EV) and silenced sidA (AsSid4 and AsSid5) strains were grown in iron limited MMcM for 5 days. Following, the O-CAS plate assay was performed. The presence of secreted siderophores induces the formation of an orange halo. b After 5 days of growth in iron limited MMcM liquid medium or in MMcM added of different concentrations of inorganic iron, the percentage of siderophores produced by different strains was verified by the CAS liquid assay. Control of the reaction was performed by incubating the culture medium with CAS without fungal cells. The presence of siderophores was compared spectrophotometrically with the values obtained in the control by the values obtained from the supernatant of the different cultures. The results were presented in percentage. Statistical analysis was performed by Student’s t test. Error bars represent standard deviation of three experimental replicates while *** demonstrates p ≤ 0.0005, ** p ≤ 0.005 and * p ≤ 0.05