Fig. 2

Maximum likelihood tree including all long read ASVs from the current study and publicly available environmental and reference sequences of Archaeorhizomycetes covering at least two of the rDNA regions ITS1, ITS2 and LSU. The tree is limited to environmental sequences that cluster on well-supported basal nodes with long read ASV sequences from the current study. Nodes represent 76 species hypothesis (SH) based on Maximum likelihood solutions in bPTP. Nodes including ASVs from the current study are cartooned to visualize how many sequences are included and labeled with SH_number according to Fig. 1, followed by ML support value for species delineation based on bPTP in parenthesis. Names for two described and two novel species are included in bold for their corresponding SHs. Following addition of environmental sequences, three local SHs split, as indicated by adding:1 and:2 after the SH number. SHs including only publicly available sequences are collapsed. Placement of previously published sequences from the studied field station is indicated by *. Bootstrap supports are calculated from 1000 iterations and indicated only when 80 or higher on branches leading up to SH from the current study. Full set of support values and sequence names are available in Fig. S10