Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

IMA Fungus

Table 2 PCR and sequencing primers used in this study

Locus	Primer	References	Sequence (5′-3′)^a	Tm
MAT1–1-1	EMAT1–1 L	This study	TGAGTCCGTTATGATTCTGG	50 °C
MAT1–1-1	EMAT1–1R	This study	GGACCATTCGCTTTCTCATA	50 °C
MAT1–2-1	EMAT1–2 L	This study	GATATGCTCACCAACCGTAA	50 °C
MAT1–2-1	EMAT1–2R	This study	TACGATCGAATAATGGCTCC	50 °C
EF-1a	EF-595F	Kauserud and Schumacher (2001)	CGTGACTTCATCAAGAACATG	50 °C
EF-1a	EF-1R	Du et al. (2012)	GGARGGAAYCATCTTGACGA	50 °C
ITS	ITS4	White et al. (1990)	TCCTCCGCTTATTGATATGC	50 °C
ITS	ITS5	White et al. (1990)	GGAAGTAAAAGTCGTAACAAGG	50 °C
F1	F1F	Du et al. (2016)	GGCTAAGATACGAGCTACGAGA	49 °C
F1	F1R	Du et al. (2016)	ACATCAATGAGAGCCATTCG	49 °C
IGS	IGSYL	This study	CTTACTCCTGCAATCGTAGT	49 °C/50 °C
IGS	IGSYR	This study	TGGTTACCCTGCCTCCAT	49 °C/50 °C

ISSN: 2210-6359