Fig. 4From: A novel Arabidopsis phyllosphere resident Protomyces species and a re-examination of genus Protomyces based on genome sequence dataNuclear marker gene phylogenetic trees. Phylogenetic trees of species in the genera Protomyces and Taphrina with the nuclear gene markers rbp2 (RNA polymerase subunit 2), tef1 (translation elongation factor 1), rbp1 (RNA polymerase subunit 1), tub2 (tubulin beta), act1 (actin 1) and concatenated sequence of the five nuclear marker genes above. Protein sequences from the respective marker genes in Schizosaccharomyces pombe were used as a query for BLASTp searches against protein sequences from Protomyces and Taphrina genome annotations. DNA sequences of each marker were then collected and aligned with ClustalX2 to construct neighbour-joining trees with 1000 bootstraps. Bootstrap support values (%) are indicated at each node. Schizosaccharomyces pombe was used as an outgroupBack to article page