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Table 1 Specimens and sequences used for the molecular phylogenetic analyses Sequences newly generated for this study are designated in bold. Specimens with an asterisk (*) correspond to the sequences with an asterisk in the same taxa. Specimens used only for supplementary nLSU phylogenetic analyses (Fig. S1) are designated with double asterisks (**)

From: Multilocus phylogenies reveal three new truffle-like taxa and the traces of interspecific hybridization in Octaviania (Boletaceae, Boletales)

Taxon Voucher No. Locality ITS 28S TEF1 RPB1
Chamonixia caespitosa KPM-NC 18071 Japan, Nagano Pref., Mts. Yatsugatake KP222908 MT812734 MT874820 MT868871
Chamonixia caespitosa KRA F-2013-38 Poland, Gorce Mts. KT001255 MT812735 MT874821 MT868872
Leccinellum aff. griseum KPM-NC 17831 Japan, Hyogo Pref., Uwano Town KC552008 JN378508 JN378449 MT868816
Leccinellum aff. griseum KPM-NC 24518 (MO455) Japan, Tochigi Pref., Nikko City MT812705 MT874790 MT868817
Leccinellum albellum KUO-07241101/FLAS-F-61741* USA, North Carolina; *USA, North Carolina, Buncombe Co. MH488723* MK601746 MK721100
Leccinellum crocipodium s.l. KPM-NC 18041 Japan, Tottori Pref., Yazu Town KC552053 KC552094 MT868818
Leccinellum corsicum Buf4507 USA KF030347 KF030435 KF030389
Leccinellum quercophilum M Kuo 07120801 USA, Illinois, Coles Co., Charleston KC691207 KC691208 MK721178
Leccinellum sp. HKAS 50221 China JQ928612 JQ928624 JQ928583 JQ928593
Leccinum aff. aurantiacum HKAS 57390 China, Yunnan Prov. JQ928625 JQ928581 JQ928591
Leccinum aff. schistophilum KPM-NC 17841 Japan, Hyogo Pref., Uwano Town KC552011 KC552055 KC552096 MT868874
Leccinum monticola HKAS 76669 China, Jilin Province, Yanbian KF112443 KF112249 KF112592
Leccinum quercinum HKAS 63502 China, Yunnan Province, Lijiang KF112444 KF112250 KF112593
Leccinum scabrum KPM-NC 17840 UK, Scotland, Burn O′ Vat KC552012 JN378515 JN378455 MT868875
Leccinum subradicatum KPM-NC 24518 Japan, Tochigi Pref., Nikko City MT934814 MT812736 MT874822 MT868873
Leccinum variicolor HKAS 57758 China, Yunnan Province, Lijiang KF112445 KF112251 KF112591
Leccinum versipelle KPM-NC 18036 UK, Scotland MT812737 MT874823 MT868876
Leccinum violaceotinctum CFMR BZ-1676 BOS-327 Belize MN250203 MK601779 MK721133
Leccinum violaceotinctum CFMR BZ-3169 BOS-616 Belize MN250215 MK601780 MK721134
Octaviania tenuipes sp. nov. KPM-NC 28187 Japan, Tokyo Met., Hachioji City, Mt. Takao MT812719 MT874805 MT868852
Octaviania tenuipes sp. nov. KPM-NC 27956 Japan, Chiba Pref., Katsuura City, Okitsu MT934803 MT812720 MT874806 MT868853
Octaviania tenuipes sp. nov. KPM-NC 27957 Kanagawa Pref., Hakone Town, Hakone-Yumoto MT812721 MT874807
Octaviania tenuipes sp. nov. KPM-NC 25370 Kanagawa Pref., Odawara City, Iryuda MT934804 MT812722 MT874808 MT868854
Octaviania tenuipes sp. nov. KPM-NC 26008 Japan, Tokyo Met., Hachijo Isl. MT934805 MT812723 MT874809 MT868855
Octaviania tenuipes sp. nov. KPM-NC 27960 Japan, Miyazaki Pref., Miyazaki City MT812724 MT874810 MT868856
Octaviania tenuipes sp. nov. KPM-NC 27965 Japan, Miyazaki Pref., Nichinan City MT934806 MT812725 MT874811 MT868857
Octaviania tenuipes sp. nov. KPM-NC 27968 Japan, Kagoshima Pref., Tarumizu City, Mt. Tohken MT934807 MT812726 MT874812 MT868858
Octaviania tenuipes sp. nov. KPM-NC 27972 Japan, Miyazaki Pref., Aya Town MT934808 MT812727 MT874813 MT868859
Octaviania tenuipes sp. nov. KPM-NC 24889 Japan, Kagoshima Pref., Tanegashima Isl. MT934809 MT812728 MT874814 MT868860
Octaviania tenuipes sp. nov. KPM-NC 24891 Japan, Kagoshima Pref., Tanegashima Isl. MT934810 MT812729 MT874815 MT868861
Octaviania tenuipes sp. nov. KPM-NC 27932 Japan, Kagoshima Pref., Yakushima Isl. MT812730 MT874816 MT868862
Octaviania tenuipes sp. nov. KPM-NC 17813 Japan, Kagoshima Pref., Amami-oshima Isl. JQ619176 JN378487 JN378429 MT868863
Octaviania tomentosa sp. nov. KPM-NC 27955 Japan, Kanagawa Pref., Minamiashigara City MT934797 MT812713 MT874799 MT868842
Octaviania tomentosa sp. nov. KPM-NC 27945 Japan, Kanagawa Pref., Minamiashigara City MT934798 MT812714 MT874800 MT868843
Octaviania tomentosa sp. nov. KPM-NC 27952 Japan, Tochigi Pref., Sano City, Mt. Karasawa MT934799 MT812715 MT874801 MT868844
Octaviania tomentosa sp. nov. KPM-NC 27954 Japan, Ibaraki Pref., Kasama City, Mt. Sashiro MT934800 MT812716 MT874802
Octaviania tomentosa sp. nov. KPM-NC 23934 Japan, Kagoshima Pref., Amami-oshima Isl., Uken-son Village MT934796 MT812712 MT874798 MT868841
Octaviania asterosperma var. potteri
= O. potteri stat. nov.
OSC 131925 USA, Florida, Wakulla Co., Skipper Bay road, St Marks NW refuge. MT934792 JN378499 JN378441 MT868835
Octaviania asterosperma var. potteri
= O. potteri stat. nov.
KPM-NC 17827 (RH30) USA, Iowa, Story County, Ames, YMCA woods JN378500 JN378442 MT868836
Octaviania asterosperma var. potteri
= O. potteri stat. nov.
KPM-NC 18032 Japan, Hokkaido, Tomakomai City MT934795 MT812710 MT874796 MT868840
Octaviania asterosperma var. potteri
= O. potteri stat. nov.
KPM-NC 17828 (RH1181) USA, Minnesota, Fillmore County, Forestville State Park. MT934793 JN378501 JN378443 No. 1 (seq1): MT868837
No. 2 (seq2): MT868838
Octaviania asterosperma var. potteri
= O. potteri stat. nov.
HUA 222100 (AC-1036) Colombia, Cundinamarca Province MT934794 MT812711 MT874797 MT868839
Octaviania potteri (registered as “Octaviania asterosperma”)** FH-284316 (RH3) USA, Iowa MK601795
Octaviania durianelloides KPM-NC 17829 Japan, Kanagawa, Minamiashigara City JQ619177 JQ619188 KJ001079 MT868865
Octaviania durianelloides KPM-NC 18031 Japan, Hokkaido, Tomakomai City MT934811 MT812731 MT874817 MT868864
Octaviania durianelloides KPM-NC 28183 Japan, Yamaguchi Pref., Mt. Sobagatake MT934812 MT812732 MT874818 MT868866
Octaviania durianelloides KPM-NC 27371 Japan, Kanagawa, Odawara City, Kuno MT934813 MT812733 MT874819 MT868867
Octaviania hesperi KPM-NC 17792 Japan, Tokyo, Hachioji City JN378479 JN378421 MT868832
Octaviania hesperi KPM-NC 17793 Japan, Kanagawa Pref., Zushi City JQ619173 JN378480 JN378422 MT868833
Octaviania hesperi KPM-NC 28189 Japan, Kanagawa Pref., Hayama-mati MT934791 MT812709 MT874795 MT868834
Octaviania japonimontana KPM-NC 17798 Japan, Tottori Pref., Kofu Town, Kagamiganaru JN378482 JN378424 MT868845
Octaviania japonimontana KPM-NC 17797 Japan, Akita Pref., near Lake Towada JQ619174 JN378483 JN378425 MT868846
Octaviania japonimontana KPM-NC 17806 Japan, Tottori Pref., Mt. Daisen JN378484 JN378426 MT868847
Octaviania japonimontana KPM-NC 17810 Japan, Tottori Pref., Yazu Town JQ619175 JN378485 JN378427 MT868848
Octaviania japonimontana KPM-NC 17812 Japan, Okayama Pref., Kagamino Town JN378486 JN378428 MT868849
Octaviania japonimontana KPM-NC 27622 Japan, Kanagawa Pref., Tanzawa Mountains MT934801 MT812717 MT874803 MT868850
Octaviania japonimontana KPM-NC 27623 Japan, Kanagawa Pref., Tanzawa Mountains MT934802 MT812718 MT874804 MT868851
Octaviania kobayasii KPM-NC 17785 Japan, Nara Pref., Mt. Kasuga JQ619170 JN378478 JN378420 MT868829
Octaviania kobayasii KPM-NC 17783 Japan, Kyoto Pref., Uji City JQ619171 JN378477 JN378419 MT868830
Octaviania kobayasii KPM-NC 28188 Japan, Kanagawa Pref., Yokohama City, Minato-ku MT934790 MT812708 MT874794 MT868831
Octaviania etchuensis KPM-NC 17822 Japan, Toyama Pref., Nakashingawa-gun, Teteyama Town JQ619182 JN378492 JN378433 MT868870
Octaviania yaeyamaensis KPM-NC 17818 Japan, Okinawa Pref., Ishigaki Isl. JQ619179 JN378490 JN378431 MT868868
Octaviania yaeyamaensis KPM-NC 17819 Japan, Okinawa Pref., Ishigaki Isl. JQ619180 JN378491 JN378432 MT868869
Octaviania asterosperma s. str.** IC1091316 Spain, Cantabria KX756591
Octaviania arbucalensis** AH-43987 Spain, Zamora KF154254
Octaviania nonae KPM-NC 17748 Japan, Kagoshima Pref., Amami-oshima JN257985 JN378459 JN378403 MT868819
Octaviania nonae KPM-NC 17752 Japan, Hiroshima Pref., Hiroshima City, Higashi-ku JN257989 JN378463 JN378407 MT868820
Octaviania decimae KPM-NC 17763 Japan, Kyoto Pref., Mt. Hiei, JN257991 JN378465 JN378409 MT868821
Octaviania celatifilia KPM-NC 24872 Japan, Kagoshima Pref., Takakuma Ravine MT934785 MT812706 MT874791 MT868823
Octaviania mortae KPM-NC 17771 Japan, Kyoto Pref., Nanzen-ji Shrine JN257995 JN378471 JN378414 MT868822
Octaviania asahimontana KPM-NC 17824 Japan, Hokkaido, Mts. Daisetsu JQ619178 JN378489 JN378430 MT868828
Octaviania cyanescens OSC 58498 Canada, British Columbia, Vancouver Island MT934789 JN378503 JN378439 MT868827
Octaviania depauperata var. depauperata** JMV951116–2 Spain, Cataluña KX756589
Octaviania depauperata var. laurarum** IC24081315 Spain, Cantabria KX756587
Octaviania lutea AQUI 3899 Italy, Provincia L’Aquila, Comune di Cappadocia KC552052 KC552093 MT868825
Octaviania mutabilis KRA F-2012-99 Poland, Beskid Niski Mts. MT934787 MT812707 MT874793 MT868826
Octaviania mutabilis** IC14081321 Spain, Cantabria KX756594
Octaviania tasmanica MEL 2341996 Australia, Tasmania MT934786 JN378495 MT874792 ← JN378436 MT868824
Octaviania zelleri MES270 USA, Maine, Tunk Lake, off route 182 MT934788 JN378498 JN378440
Rossbeevera bispora KPM-NC 28186 China, Guangdong Province, Dinghu District MT934784 MT812704 MT874788 MT868814
Rossbeevera cryptocyanea KPM-NC 26877 Japan, Okinawa Pref., Kume-jima Isl. MT934783 MT812703 MT874787 MT868813
Rossbeevera eucyanea KPM-NC 28182 Japan, Yamaguchi Pref., Mt. Sobagatake MT934782 MT812702 MT874786 MT868812
Rossbeevera griseovelutina TNS-F-36990 Japan, Hyogo Pref. HQ693876 HQ693881 KC552074 MT868810
Rossbeevera griseovelutina TNS-F-36991 Japan, Okayama Pref. KC551985 KC552032 KC552077 MT868811
Rossbeevera pachydermis KPM-NC 23336 New Zealand, NZ North Isl., Te Urewera National Park KJ001088 KJ001095 KP222912 MT868809
Rossbeevera paracyanea KPM-NC 18087 Japan, Nara Pref., near Mt. Kasuga KJ001086 KJ001100 MT874789 ← KJ001082 MT868815
Rossbeevera vittatispora MEL 2128491 Australia, New South Wales MT934781 KX685725 KX685719 MT868808
Rossbeevera vittatispora MEL 2329434 Australia, Victoria, Midlands KJ001084 KJ001097 KJ001075 MT868807
Rossbeevera yunnanensis KPM-NC 17850 China, Yunnan Prov., Chu Xang Pref., Mt. Zi Xi KC551990 JN979437 KC552080 MT868806
Turmalinea mesomorpha subsp. mesomorpha KPM-NC 18014 Japan, Iwate Pref., Appi-Kogen KC552000 KC552048 KC552091 MT868804
Turmalinea mesomorpha subsp. sordida KPM-NC 17743 Japan, Ehime Pref., Matsuyama City, Mt. Takanawa KC552002 KC552050 KJ001078 MT868805
Turmalinea persicina KPM-NC 18001 Japan, Kyoto Pref., Iwakura KC551991 KC552038 KC552082 MT868802
Turmalinea yuwanensis KPM-NC 18011 Japan, Kagoshima Pref., Amami-Ohshima Isl. KC551998 KC552046 KC552089 MT868803
Retiboletus fuscus HKAS 59460 China, Yunnan Prov. JQ928613 JQ928626 JQ928580 JQ928590
Retiboletus griseus Both sn USA, New York KF030308 KF030414 KF030373
Borofutus dhakanus HKAS 73792 Bangladesh, Dhaka Division, Gazipur, Bhawal National Park JQ928607 JQ928617 JQ928575 JQ928587
Spongiforma thailandica DED 7873 Thailand, Nakorn Nayok Province, Khao Yai National Park EU685113 EU685108 KF030436 KF030387
Tylocinum griseolum HKAS 50281/HKAS 50209* China, Yunnan Prov., Dadugang Town KF112451 KF112284 KT990919*
Spongispora temasekensis HKAS 101385 Singapore, Singapore Botanic Gardens MG979395 MG672512 MG674377 MG979393
Binderoboletus segoi BRG 41206 Guyana, Region 8 Potaro-Siparuni, Pakaraima Mountains LC043078 LC043078 LC043079