Fig. 5

Maximum Likelihood phylogram of Macrophomina species inferred from the concatenated alignment of the internal transcribed spacer sequences of the nuclear ribosomal DNA and the intervening 5.8S region (ITS), actin, calmodulin, translation elongation factor one-alpha, and beta tubulin sequences obtained from Poudel et al. (2021) and Sarr et al. (2014). Alignment and tree were submitted to TreeBase (No. 28996). The tree was constructed using RAxML v.8 (Stamatakis 2014), based on the GTR substitution model with gamma-distribution rate variation for individual partitions. The tip labels in bold represent ex-type strains, and asterisks denote strains sequenced in the current study. Bootstrap support values > 80% are shown at the branches. The tree is rooted to Botryosphaeria dothidea (Poudel et al. 2021). The scale bar represents nucleotide substitutions per site