Table 2 Primers used for DNA amplification of Cyathus species
Locus | Primer name | Sequence (5′ → 3′) | Sense | References |
|---|---|---|---|---|
nrITS | ITS1 | TCCGTAGGTGAACCTGCGG | F | White et al. (1990) |
ITS1F | CTTGGTCATTTAGAGGAAGTAA | F | Gardes and Bruns (1993) | |
ITS2 | GCTGCGTTCTTCATCGATGC | R | White et al. (1990) | |
ITS4 | TCCTCCGCTTATTGATATGC | R | White et al. (1990) | |
ITS-CyR3 | ACCYAATAGAAGCRGYHCAA | R | This study | |
ITS-CyF4 | ADTTGAWGTCRGCTYTCKCTG | F | This study | |
28S (nrLSU) | LR0R | ACCCGCTGAACTTAAGC | F | Vilgalys and Hester (1990) |
LR5 | TCCTGAGGGAAACTTCG | R | Vilgalys and Hester (1990) | |
LSU-CyR | ATGCCAACATCCGAAGCAC | R | This study | |
LSU-CyF | AAGGGAAACGCTGGAAGTCA | F | This study |