Fig. 6

set1 disruption results in a reduction of polarity index and ability to induce macrophage death. a Polarity index of the wild-type MU636 (WT) and set1 deletion strains determined from phagocytosed spores by mouse macrophages (J774A.1) after 5.5 h of incubation. b Images of live-cell microscopy of mouse macrophages (Φ) co-cultured for 24 h with spores of WT and set1∆ strains. The dead macrophages were stained with PI. Macrophage non-interacting with spores served as a control. c Macrophages cell death at 24 h of interaction with M. lusitanicus spores estimated by determining percentage of PI-stained macrophages in the fluorescent images. The charts display means ± SD based on three biological repetitions. The statistical differences were obtained by one-way ANOVA and indicated by asterisks (****P < 0.0001)