Fig. 6
From: Molecular techniques for pathogen identification and fungus detection in the environment
Schematic representation of the mechanism of LAMP. A. General location of the LAMP primer set in relation to defined regions of the target DNA. Forward (F3) and backward (B3) outer primers and forward (FIP) and backward (BIP) inner primers are indicated. B, C. Basic principle and amplification steps in LAMP. In general new DNA strands are synthesizes from the F3 and B3 primers, and these strands are recognized by FIP and BIP to start loop-mediated autocycling amplifications. The final products are stem-loop DNAs with several inverted repeats of the target DNA, and cauliflower-like structures bearing multiple loops (modified from diagrams at <http://loopamp.eiken.co.jp/e/lamp/principle.html> © Eiken Chemical.