Table 1 Growth media and detection reagents used for substrate degradation assays.
From: Phialosimplex salinarum, a new species of Eurotiomycetes from a hypersaline habitat
Test substrate | Growth medium | Detection reagent |
|---|---|---|
Starch | 0.8% peptone; 0.5% NaCl; 0.6% sol. starch; 1.2% agar; H2O | Lugolâs solution |
Chitin | 0.8% peptone; 0.5% NaCl; 0.6% colloidal chitin; 1.2% agar; H2O | none |
Cellulose | 0.8% peptone; 0.5% NaCl; 0.6% carboxymethyl cellulose; 1.2% agar; H2O | Congo red; 0,5 M HCl |
Peptides and protein | 0.8% nutrient broth; 1.3% powdered milk; 1.2% agar; H2O; pH 7.2 | none |
Lipid | 0.8% peptone; 0.5% NaCl; 1% Tween 80; 1.2% agar; H2O | none |
Lignin | 1% malt extract; 0.4% yeast extract; 0.4% glucose; 1.2% agar; H2O | 0.2% K2HPO4; 0.2% KCl; 0.2% MgSO4·7 H2O; 2% agar; ABTS 1 mM; H2O; pH 5 |