Table 2 Status of Phytophthora alticola isolates submitted to different culture collections.
Isolate | Sequence 1 | Notes on status of isolate |
|---|---|---|
CMW 19416 PREM 59214-paratype | no sequence (OD) | Lost in CMW collection. Only papillate, caducous sporangia and chlamydospores observed from preserved slide associated with PREM 59214 |
CMW 19417 PREM 59215-holotype CBS 121937 | no sequence (OD) ITS, CO, YPT1, TEF (q-bank) | Lost in CMW collection. Supposed corresponding isolate in CBS is actually P. palmivora and all sequence data on q-bank associated with this isolate is P. palmivora. Only papillate, caducous sporangia and chlamydospores observed from preserved slide associated with PREM 59215 |
CMW 19419 PD 01642 | ITS and BT (OD) | Lost in CMW collection |
CMW 19421 PD 01641 | ITS and BT (OD) | Lost in CMW collection |
CMW 19422 PD 01640 CMW 19423 | ITS and BT (OD) | Lost in CMW collection |
PD 01639 | ITS and BT (OD) | Lost in CMW collection |
CMW 19424 PREM 59216-paratype CBS 121938 PD 01638 | ITS and BT (OD) | Lost in CMW collection. Sequence on q-bank of ITS and BT is from the original description. The ITS of isolate re-sequenced in this study corresponds to P. frigida. Only aplerotic oospores and amphigynous antheridia observed from preserved slide associated with PREM59216 |
CMW 19425 PREM 59217-paratype CBS 121939 PD 01637 | ITS and BT (OD) ITS, CO, YPT1, TEF (q-bank) | Living in CMW collection and renamed CMW 35429. ITS and BT of re-sequenced isolate differ from original description by 3 and 2 bp respectively. ITS and CO sequence on q-bank is identical to sequence of isolate CMW 35429 obtained in the current study. Only aplerotic oospores and amphigynous antheridia observed from preserved slide associated with PREM 59217 |
CMW 35429 P16948 | ITS, cox1, ENO, HSP, BT ITS (GA) | Was sent to WPC as CMW 35429 as a replacement for P. alticola and named WPC 16948. ITS sequence supplied by Gloria Abad is identical to that obtained in the current study for isolate CMW 35429 |
PD 01914 P16053 | cox2 and cox1 (PD) | Was sent to WPC as P. alticola isolate CMW 19424 but when sequenced it was identified as being an isolate of P. frigida |
PD 02043 P16051 | cox2 and cox1 (PD) | Was sent to WPC as P. frigida isolate CMW 19433 and when sequenced it was identified as being an isolate of P. frigida |
PD 02044 P16054 | cox2 and cox1 (PD) | Was sent to WPC as P. alticola isolate CMW 19425 but when sequenced it was identified as being an isolate of P. frigida |
P16052 | cox1 (PD) | Was sent to WPC as P. frigida isolate CMW 20311 but when sequenced it was identified as being an isolate of P. alticola and thus cannot be linked to any isolate from CMW collection |
VHS 26631 P19861 | ITS, cox1, ENO, HSP, BT | List in WPC as a neotype for P. alticola, but this is not recommended as the isolate is from a different host and a different country from the original description. In current study this is considered an isolate of P. boodjera. |