Table 1 List of primers used for PCR amplification of small subunit (SSU), internal transcribed spacer (ITS), and large subunit (LSU) rDNA.
Locus | Primer name | Sequence | Reference | |
|---|---|---|---|---|
SSU rDNA | NS1 | forward | GTAGTCATATGCTTGTCTC | White et al. 1990 |
SSU rDNA | NS2 | reverse | GGCTGCTGGCACCAGACTTGC | White et al. 1990 |
SSU rDNA | NS4 | reverse | CTTCCGTCAATTCCTTTAAG | White et al. 1990 |
SSU rDNA | SL344 | forward | GGTCGCAAGGCTGAAACTTA | Landvik et al. 1997 |
SSU rDNA | NS6 | reverse | GCATCACAGACCTGTTATTGCCTC | White et al. 1990 |
SSU rDNA | SL122 | forward | AGGCGCGCAAATTACCCAAT | Landvik et al. 1997 |
SSU rDNA | SR4 | reverse | AAACCAACAAAATAGAA | R. Vilgalys unpublished |
SSU rDNA | NSL1 | forward | GTAGTGTCCTCrCATGCTTTTGAC | present study |
SSU rDNA | NSL2 | reverse | AATCyAAGAATTTCACCTCTGAC | present study |
SSU rDNA | L | forward | AACCTGGTTGATCCTGCCAGT | Wrzosek 2000 |
SSU rDNA | 402 | forward | GCTACCACATCCAAGGAAGGCA | Wrzosek 2000 |
SSU rDNA | 416 | reverse | ATTTGCGCGCCTGCTGCCTTCC | Wrzosek 2000 |
SSU rDNA | 895 | forward | GTCAGAGGTGAAATTCTTGGAT | Wrzosek 2000 |
SSU rDNA | 898 | reverse | TAAATCCAAGAATTTCACCTCT | Wrzosek 2000 |
SSU rDNA | 1144 | forward | GCCTGCGGCTTAATTTGACTCAACA | Wrzosek 2000 |
SSU rDNA | 1308 | reverse | CTCGTTCGTTAACGGAATTAACC | Wrzosek 2000 |
SSU rDNA | R | reverse | TGATCCTTCTGCAGGTTCACCTACG | Wrzosek 2000 |
ITS rDNA | ITS1f | forward | CTTGGTCATTTAGAGGAAGTAA | Gardes & Bruns 1993 |
ITS rDNA | ITS4 | reverse | TCCTCCGCTTATTGATATGC | White et al. 1990 |
ITS rDNA | ITS4_kyo1 | reverse | TCCTCCGCTTWTTGWTWTGC | Toju et al. 2012 |
ITS rDNA | ITS5 | forward | GGAAGTAAAAGTCGTAACAAGG | White et al. 1990 |
ITS rDNA | ITS2 | reverse | GCTGCGTTCTTCATCGATGC | White et al. 1990 |
LSU rDNA | LR0R | forward | ACCCGCTGAACTTAAGC | R. Vilgalys unpublished |
LSU rDNA | LR1R | forward | AGGAAAAGAAACCAACC | Moncalvo et al. 1993 |
LSU rDNA | LIC24R | forward | GAAACCAACAGGGATTG | Miadlikowska & Lutzoni 2000 |
LSU rDNA | LR3 | reverse | GGTCCGTGTTTCAAGAC | Vilgalys & Hester 1990 |
LSU rDNA | LR5 | reverse | ATCCTGAGGGAAACTTC | Vilgalys & Hester 1990 |
LSU rDNA | LR7 | reverse | TACTACCACCAAGATCT | Vilgalys & Hester 1990 |
LSU rDNA | NL1 | forward | GCATATCAATAAGCGGAGGAAAAG | Kurtzman & Robnett 1997 |
LSU rDNA | NL4 | reverse | GGTCCGTGTTTCAAGACGG | Kurtzman & Robnett 1997 |