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Table 3 Performances of algorithms in mapping FASTQ files of different size with large reference libraries.

From: NGS barcode sequencing in taxonomy and diagnostics, an application in “Candida” pathogenic yeasts with a metagenomic perspective

Library

Algorithm

FASTQ

Time Performances

 

Mapping parameters

 

acronym

sequences

 

strain

reads

Mapping time (sec)

CPU-time (sec)

used reads

unused reads

Matches

min pairwise identities

max pairwise ideniies

 

15,565

BTL

A

21,238

63

33,34

18,274

2,964

684

74,00%

100%

CBS

  

B

58,263

99

123

54,822

3,441

661

91,00%

100%

ITS

 

BBm

A

21,238

80

39,82

18,323

2,915

626

68,20%

100%

   

B

58,263

114

49,57

55,675

2,588

708

62,30%

100%

 

34,683

BTL

A

21,238

186

104

19,163

2,075

2,051

73,40%

100%

CBS

  

B

58,263

273

393

57,140

1,123

2,457

79,50%

100%

ITS-LSU

 

BBm

A

21,238

204

109

19,000

2,238

1,939

58,80%

100%

   

B

58,263

311

161

56,930

1,333

2,445

63,60%

100%

 

2,727

BTL

A

21,238

16,7

13,59

13,904

7,334

319

74,10%

100%

ISHAM

  

B

58,263

30,3

33,5

36,416

21,847

297

82,00%

100%

ITS

 

BBm

A

21,238

37,7

16,13

13,665

7,573

280

74,50%

100%

   

B

58,263

45,2

26,03

35,653

22,610

305

85,70%

100%

  1. Strain A: CMC 1793; strain B: CMC 1818.