Fig. 4

Multiple-alignment-based phylogenetic analysis of 773 randomly selected pyrosequencing ITS reads originating from a single species, the basidiolichen former Cora inversa (after Lücking et al. 2014), with other species of Cora represented by five or more ITS Sanger sequences (from Lücking et al. 2016a). Even including all sequencing errors, the reads form a single, strongly supported clade together with ITS Sanger sequences from the same samples; however, the same reads result in multiple species estimates using a clustering approach (see Fig. 3).