Table 2 Primercombinations used during this studyforamplification and sequencing.
From: Novel primers improve species delimitation in Cercospora
Locus | Primer | Primer sequence 5′ to 3′ | Annealing temperature (°C) | Orientation | Reference |
|---|---|---|---|---|---|
Beta-tubulin (tub2) | T1 | AAC ATG CGT GAG ATT GTAAGT | 48 | Forward | O’Donnell & Cigelnik 1997 |
|  | β-Sandy-R | GCR CGN GGV ACR TAC TTG TT | 48 | Reverse | Stukenbrock et al. 2012 |
| Â | BT-1F | GTC CWC ACC GCC CCT GAT | 56 | Forward | This study |
| Â | BT-1R | CTT GTT RCC RGAAGC CTR TGS | 56 | Reverse | This study |
RNA polymerase II second largest subunit (rpb2) | fRPB2-5F | GAY GAY MGW GAT CAY TTY GG | 47 | Forward | Liu et al. 1999 |
| Â | fRPB2-414R | ACM ANN CCC CAR TGN GWR TTR TG | 47 | Reverse | Quaedvlieg et al. 2011 |
| Â | fRPB2-7cF | ATG GGY AAR CAA GCY ATG GG | 49 | Forward | Liu et al. 1999 |
| Â | fRPB2-11aR | GCR TGG ATC TTR TCR TCS ACC | 49 | Reverse | Liu et al. 1999 |
|  | RPB2-C5F | TGGGGA GAY CAR AAR AAA GC | 60→58→56 | Forward | This study |
|  | RPB2-C8R | ACG GAA TCT TCC TGG TTG TA | 60→58→56 | Reverse | This study |
Glyceraldehyde-3-phosphate dehydrogenase (gapdh) | Gpd1-LM | ATT GGC CGC ATC GTC TTC CGC AA | 60→58→53 | Forward | Myllys et al. 2002 |
|  | Gpd2-LM | CCC ACT CGT TGT CGT ACC A | 60→58→53 | Reverse | Myllys et al. 2002 |