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Table 2 Primercombinations used during this studyforamplification and sequencing.

From: Novel primers improve species delimitation in Cercospora

Locus Primer Primer sequence 5′ to 3′ Annealing temperature (°C) Orientation Reference
Beta-tubulin (tub2) T1 AAC ATG CGT GAG ATT GTAAGT 48 Forward O’Donnell & Cigelnik 1997
  β-Sandy-R GCR CGN GGV ACR TAC TTG TT 48 Reverse Stukenbrock et al. 2012
  BT-1F GTC CWC ACC GCC CCT GAT 56 Forward This study
  BT-1R CTT GTT RCC RGAAGC CTR TGS 56 Reverse This study
RNA polymerase II second largest subunit (rpb2) fRPB2-5F GAY GAY MGW GAT CAY TTY GG 47 Forward Liu et al. 1999
  fRPB2-414R ACM ANN CCC CAR TGN GWR TTR TG 47 Reverse Quaedvlieg et al. 2011
  fRPB2-7cF ATG GGY AAR CAA GCY ATG GG 49 Forward Liu et al. 1999
  fRPB2-11aR GCR TGG ATC TTR TCR TCS ACC 49 Reverse Liu et al. 1999
  RPB2-C5F TGGGGA GAY CAR AAR AAA GC 60→58→56 Forward This study
  RPB2-C8R ACG GAA TCT TCC TGG TTG TA 60→58→56 Reverse This study
Glyceraldehyde-3-phosphate dehydrogenase (gapdh) Gpd1-LM ATT GGC CGC ATC GTC TTC CGC AA 60→58→53 Forward Myllys et al. 2002
  Gpd2-LM CCC ACT CGT TGT CGT ACC A 60→58→53 Reverse Myllys et al. 2002