Skip to main content

One Fungus = One Name: DNA and fungal nomenclature twenty years after PCR


Some fungi with pleomorphic life-cycles still bear two names despite more than 20 years of molecular phylogenetics that have shown how to merge the two systems of classification, the asexual “Deuteromycota” and the sexual “Eumycota”. Mycologists have begun to flout nomenclatorial regulations and use just one name for one fungus. The International Code of Botanical Nomenclature (ICBN) must change to accommodate current practice or become irrelevant. The fundamental difference in the size of fungi and plants had a role in the origin of dual nomenclature and continues to hinder the development of an ICBN that fully accommodates microscopic fungi. A nomenclatorial crisis also looms due to environmental sequencing, which suggests that most fungi will have to be named without a physical specimen. Mycology may need to break from the ICBN and create a MycoCode to account for fungi known only from environmental nucleic acid sequence (i.e. ENAS fungi).


It has been a bit over two decades since the polymerase chain reaction (PCR) changed evolutionary biology in general and fungal systematics in particular. Even before PCR became generally available, mycologists realized that the evolutionary record contained in the nucleic acid sequence of every fungus could be used to merge two systems of nomenclature that had been employed in most fungi, i. e. one for the “Eumycota” based on sexual morphology and the “Deuteromycota” based on all other morphologies (Berbee & Taylor 1992, Bruns et al. 1991, Guadet et al. 1989, Reynolds & Taylor 1992). Why, then, has it taken more than two decades for nomenclature to catch up with biology, and why is the possibility of nomenclatorial rapprochement now being taken seriously? These questions, and three others posed to the participants in this symposium will be the subject of this contribution: Does DNA sequencing make dual nomenclature superfluous? Can the International Code of Botanical Nomenclature (ICBN) (McNeill et al. 2006) be modified to enable this process, or would a MycoCode be more effective? How can the mycological community get rid of the legacy of dual nomenclature and Article 59 without nomenclatural chaos?

Two examples illustrate the practical problems raised by dual nomenclature. First, this year, while serving as a member of a governmental committee researching the use of mycoherbicides to eradicate drug crops, it fell to me to explain the nomenclature of two poppy pathogens that are sister species, one named as a teleomorph Crivellia papaveracea and the other as an anamorph, Brachycladium papaveris (Inderbitzin et al. 2006) (Fig. 1). The fifteen other members of the committee, eleven academics and four very knowledgeable staff, stared at me in disbelief when I said that sister species could have different generic names. Second, together with Tom Bruns, I have been directing research about fungi that naturally decay plants proposed as sources of lignocellulose for the production of biofuels. In the course of this work, we have sequenced ITS using DNA isolated from the decaying grasses and compared the sequences to those deposited in GenBank. Often, a single sequence will be attached to two names; you guessed it, it’s the same fungus with some GenBank sequences having been deposited under the teleomorph name and others under the anamorph name. Perpetuation of dual nomenclature when we have the means to abandon it is hindering mycology, both scientifically and socially.

Fig. 1
figure 1

Phylogenetic relationships of the sister species Crivellia papaveracea and Brachycladium papaveris, the former named as tele-omorphic and the latter as an anamorphic fungus. The Crivellia state of B. papaveris remains unnamed due to a lack of suitable material to serve as a nomenclatural type (Inderbitzen et al. 2006).

Dual nomenclature has persisted for the past 20 years because few mycologists are deeply interested in both molecular phylogenetics and nomenclature. One Fungus = One Name has gained momentum, as evidenced by this conference, because mycologists who are studying the molecular phylogenetics of economically important fungal groups have begun naming newly recognized genus-level clades with just one Ascomycota name, whether or not the fungus exhibits sexual reproduction. The first thorough exploration of this practice was provided by Crous et al. (2006), whose revision of the Botrysphaeriaceae includes this sentence, “Separate teleomorph and anamorph names are not provided for newly introduced genera, even where both morphs are known.” Where a teleomorph name was available, as in the case of Botryosphaeria, the authors used it. Where only anamorph names were available, they were used, e.g. Macrophomina or Neoscytalidium. Where a new clade was segregated from an existing teleomorph genus, and best distinguished by the anamorphic morphology, the new name reflected the anamorph, e.g. Neofusicoccum.

Matters were taken further in a study of Penicillium species by Houbraken et al. (2010). As they put it, “Using this approach and applying the concept of one name for one fungus (Reynolds & Taylor 1992), we have chosen to describe these two species under [their] anamorphic name.” That is, Houbraken et al. described new species that have both anamorphic and teleomorphic states as species in the anamorph-typified genus Penicillium and ignored the existing teleomorphic generic name, Eupenicillium. These actions are clearly outside the ICBN and constitute a social rebellion. Though smaller and far less important than social rebellions concerning, for example, women’s rights, the rights of African Americans, or those of homosexuals, this mycological rebellion is similar to the others in that activism has outpaced the law and the law must now change or become irrelevant.

Dual nomenclature has a long history. The choice made by Houbraken et al. (2010) to use the anamorph name Penicillium over the teleomorph name Eupenicillium for one of the most economically important fungi echoes the choice made more than 40 years earlier by Raper & Fennel (1965) when they applied the anamorphic name Aspergillus to all members of that genus whether or not the species also produced a sexual structure. Forty years are not enough to understand the origins of dual nomenclature, to do that we have to go all the way back to Linnaeus and the beginning of botanical nomenclature. In this tour back through time, our guides will be Weresub & Pirozynski through their excellent article on the history of fungi that produce both meiotic and mitotic spores, that is, pleomorphic fungi (Weresub & Pirozynski 1979) and the opening chapters of Selecta Fungorum Carpologia, the monumental work of Louis-René Tulasne and Charles Tulasne (Fig. 2) (Tulasne & Tulasne 1861).

Fig. 2
figure 2

Louis Renè Tulasne (I) and Charles Tulasne (r). Photo: courtesy of the National Museum of Natural History, Paris.

The Tulasne’s point out that Linnaeus based his plant taxonomy on floral morphology and that he could demonstrate that each plant had but one type of flower. At a time when fungi were considered to be plants, and fungal spores were equated with seeds, Linnaeus extended his taxonomic concept to fungi. The Tulasne brothers then argue that Linnaeus had such an influence over his mycological contemporaries, Fries foremost among them, that these mycologists were in denial about pleomorphy, despite their being able to see more than one type of “seed” through their lenses.

“In the Mucedinei [Fries] sees the conidia… but everywhere he flatly denies that there occur “two kinds of sporidia on the same plant”, exactly as if he had heard, sounding in his ears, the loud voice of Linnaeus, crying “It would be a remarkable doctrine - that there could exist races differing in fructification, but possessing one and the same nature and power; that one and he same race could have different fructifications; for the basis of fructification, which is also the basis of all botanical science, would thereby be destroyed, and the natural classes of plants would be broken up” (Tulasne & Tulasne 1861: 48Footnote 1). The brothers go on to chide Linnaeus, adding “But since the illustrious author always completely abjured the use of magnifying glasses, and therefore scarcely ever tried to describe accurately either conidia or spores, we fear (may he pardon the statement) that he really knew very few seeds of either kind” (Tulasne & Tulasne 1861: 48–49). The influence that the size of an organism has on its systematics can be profound (Taylor et al. 2006). The fact that the overwhelming majority of plants are macroscopic while the overwhelming majority of fungi are microscopic still affects nomenclature and will be revisited nearthe end of this article.

Louis René and Charles Tulasne went on to argue against mycological denial of pleomorphy when they wrote, “The fungus upon which we are now touching [Pleospora] is not only almost the commonest of all belonging to its order, but also affords a wonderful proof of our doctrine concerning the multiple nature of the seeds ofspecies offungi” (Tulasne & Tulasne 1861: 248). One cannot help wondering if the brothers guessed not only that their work was controversial, but that the mycological world was heading toward dual nomenclature, when they wrote, “As today we have seen the various members of the same species now unwisely torn from one another against the laws of nature …” (Tulasne & Tulasne 1861: 189).

Alas, when the most useful characters that could be used for classification were meiosporic, and when many fungi did not exhibit them, there were not many options and the one that prevailed was dual nomenclature. Fuckel, a retired pharmacist, got the ball rolling (Fuckel 1870) and Saccardo did the heavy lifting with his Sylloge Fungorum beginning in 1882 (Saccardo 1882). By 1910, the International Rules of Botanical Nomenclature (Briquet 1912) contained a section of Article 49, Art 49 bis (the precursor of the current Article 59), that forbade “botanical” names for any but the sexual stage of pleomorphic fungi and that is where matters rest with the current ICBN.

Saccardo’s use of mature anamorph morphology is wonderfully convenient for classification and identification but, obviously, it is not based on evolutionary relationships. The hope that study of mitospore development would lead to a separate systematics based on evolutionary relationships began with Vuillemin (1910a, b) and Mason (1933, 1937) and led to the work of Hughes (1953), Tubaki (1958) and Barron (1968). Elegant microscopic studies of mitospore development followed (Cole & Samson 1979) and the movement reached its zenith at the second Kananaskis conference (Kendrick 1979). Just as these studies of development were peaking, two events occurred in the realms of evolution and systematics that promised the irresistible appeal of a new approach and a seemingly endless supply of characters - cladistic analysis (Hennig 1966) and access to nucleic acid variation.

The first applications of nucleic acid variation to fungal systematics involved DNA-DNA hybridization of yeasts (Kurtzman 1980) and then sequencing of nucleic acids. Pioneering work with painfully difficult RNA sequencing modeled on the work of bacteriologists (Walker & Doolittle 1982, 1983) was followed by DNA sequencing (Gottschalk & Blanz 1984, Guadet et al. 1989, Gueho et al. 1989). But it was the discovery of the polymerase chain reaction (PCR) (Rabinow 1996, Saiki et al. 1988) that made possible the broad studies we nowtake for granted.

The first application of PCR amplified DNA sequence to fungal phylogenetics demonstrated the evolution of hypogeous fungi from mushroom ancestors (Bruns et al. 1989; Fig. 3). This work relied on the development of primers designed to amplify regions of both mitochondrial and nuclear rDNA including the nuclear small subunit, large subunit and internal transcribed spacer (ITS), which were published the following year and have been cited a bit more often than once-a-day since then (White et al. 1990; Fig. 4).

Fig. 3
figure 3

Phylogenetic analysis of PCR amplified rDNA showing the evolution of hypogeous Basidiomycota in the genus Rhizopogon, from mushroom ancestors in the genus Suillus (Bruns et al. 1989). Adapted from Bruns et al. (1989).

Fig. 4
figure 4

Authors of the publication of PCR primers for the amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Left to right: Tom Bruns, Tom White, Steve Lee, and John Taylor. Photo: taken in 2010, 20 years after the publication of White et al. (1990).

Within a few years, analysis of PCR amplified rDNA showed that the anamorphic Sporothrix schenckii nested within the teleomorph genus Ophiostoma (Berbee & Taylor 1992; Fig. 5). This work demonstrated the integration of anamorphic and teleomorphic fungi based on DNA variation, as had earlier work on Fusarium (Guadet et al. 1989). These studies showed a separate classification for “Deuteromycota” to be superfluous.

Fig. 5
figure 5

Phylogenetic analysis of PCR amplified rDNA showing the anamorphic Sporothrix schenckii nestled within the teleomorphic genus Ophiostoma (Berbee & Taylor 1992).

That same year, Reynolds & Taylor (1992) addressed the nomenclatural implications of using DNA variation to assess the phylogenetic relationships of fungi, writing, “The use of nucleic acid sequence allows systematists to demonstrate the phylogenetic relatedness of fungi possessing and lacking meiotically produced spores. … This demonstration presents a serious challenge to the separate classification of these two types of fungi and undermines the elevated position that characters associated with sexual reproduction have held in the classification of higher fungi. … We believe that all fungi should be classified in one system and that characters associated with sexual reproduction should be given the same weight as other characters. … By the broad interpretation [of Article 59] in current use, the potential for pleomorphy is assumed of all fungi and the Article is applied to all fungi. … With an alternative and strict interpretation however, Article 59 would apply only to fungal species that have been actually demonstrated to be pleomorphic. Under the latter interpretation, sexual, asexual, and pleomorphic fungi would be classified together and form taxa would not be necessary.”

Following the Fungal Holomorph Symposium in Newport (OR, USA) to discuss nucleic acid variation and the integration of anamorphic and teleomorphic classifications (Reynolds & Taylor 1993), there have been presentations and discussions on the topic at every International Mycological Congress from Vancouver (1994; Taylor 1995) to Edinburgh (2010; Norvell et al. 2010), leading up to the present One Fungus = One Name conference and the Amsterdam Declaration (Hawksworth et al. 2011).

Nucleic acid variability has proved to be useful in other areas of fungal systematics and classification related to mitotic fungi. Beginning with the mitosporic human pathogen Coccidioides immitis, DNA variation has been used to show that anamorphic fungi recombine in nature (Burt et al. 1996), that they speciate (Koufopanou et al. 1997), and that, based only on DNA variation, they can be described in the system for Ascomycota (Fisher et al. 2002). As Fisher et al. wrote when they described a new Coccidioides species as an ascomycete, “Coccidioides posadasii is morphologically indistinguishable from Coccidioides immitis. C. posadasii is diagnosed by the following nucleotide characters (given as the gene, the nucleotide position in the gene, and, parenthetically, the nucleotide fixed in C. posadasii) showing reciprocal fixation between C. immitis and C. posadasii: Chitin synthase positions 192 (A), 288 (T); Dioxygenase positions 872 (C), 1005 (C), 1020 (G), 1179 (C), 1272 (T); etc.” Of course, description is not the same as acceptance. In the case of Coccidioides posadasii, acceptance for this “Select Agent” came from an unexpected quarter, the United States Congress (Federal Register 2005).

Another point made soon after PCR became available was that DNA, or even a DNA sequence, could act as the type element in a species description (Reynolds & Taylor 1991). This observation has gained importance due to the advent of environmental sequencing, where mycologists use PCR primers for rDNA to amplify variable regions from DNA isolated from soil or plants. Environmental sequencing has begun to produce large numbers of rDNA sequences that document the existence of fungi for which there is neither a specimen nor a culture. Most importantly, ecological studes have shown that the number of these DNA-only fungi, or “Environmental Nucleic Acid Sequences” (ENAS) can exceed the number of fungi for which there is a culture or specimen (Jumpponen & Jones 2009, 2010). This imbalance poses a challenge to fungal classification and nomenclature that may dwarf the challenge of integrating anamorphic and teleomorphic fungi.

David Hibbett, in his plenary presentation at IMC9 (Hibbett et al. 2011), noted that the number of fungal OTUs added each year to GenBank that are based only on rDNA sequences (ENAS fungi) is now exceeding the number from fungi with cultures or specimens (Fig. 6). Ecologists face the prospect that most of the fungal species dwelling in their favourite environment can neither be cultivated nor collected; as a result they are going to have to rely on ENAS to assess the true fungal diversity. Each of these ecological studies may add hundreds or thousands of ENAS to GenBank. Already, searches of GenBank using a new ENAS mostly recover previously deposited ENASs, which are identified not by names but by numbers. Imagine two ecological studies, one where each new ENAS in tables or figures is associated with a numbered, existing ENAS and the other where the existing ENASs have been named - the reader would come away with ignorance on the one hand and understanding on the other. Fungal classification and nomenclature must respond to this challenge by developing a means of associating ENASs with names and the response must be timely.

Fig. 6
figure 6

Graph of the Operational Taxonomic Units (OTUs) added to GenBank from 1991 to 2009 showing the increasing proportion of OTUs based only on environmental nucleic acid sequence (ENAS). Adapted from Hibbett et al. (2011).

As discussed by Hibbett etal. (2011), fungi known only as ENAS can be named by comparison to named fungi already in GenBank. It seems important that this name be identified as attached to an ENAS rather than a culture or specimen, perhaps by appending ENAS as a suffix. Several essential issues will have to addressed before ENAS naming can begin, among them the problems of sequencing errors, variation in rDNA sequence within an individual, and accommodation of all these new ENAS fungi in MycoBank (Hawksworth et al. 2010). Perhaps most unsettlingly, the naming will have to be automated in some way because no one can possibly name the thousands of new sequences that will arise in each new environmental study.

At this point, a reader might fairly ask, if separate “Deuteromycota” and “Eumycota” nomenclatural systems still remain separate 20 years after their merger became intellectually obvious, how could anyone possibly entertain thoughts about the acceptance of the automated description of fungi based only on DNA sequence? I see two steps to acceptance of ENAS fungi. The first step would be a published demonstration of the naming of ENAS fungi, echoing the aforementioned social activism already in play for One Fungus = One Name (Crous et al. 2006, Houbraken et al. 2010). The second step, acceptance of named ENAS fungi by the ICBN, is the tougher problem and is unlikely to occur quickly enough to satisfy the pressing needs of fungal ecologists. Here, social activism alone is not going to be sufficient largely due to the problem of organismal size, mentioned above, which is as old as Linnaeus. Mycologists cannot expect botanists to fully appreciate the problems created by working with microscopic organisms that can neither be routinely collected nor cultured. Mycology, to free itself from the legacy of botanical nomenclature, needs a nomenclatorial revolution.

It is time for mycologists, who best understand the nomenclatorial needs peculiar to fungi, to design a nomenclatorial code for fungi. The timing could not be better because over the past two decades one of our own, David Hawksworth, has been helping to guide the development of the BioCode (Greuter et al. 2011, Hawksworth 2011). Modification of the draft BioCode to enable One Fungus = One Name and to accommodate ENAS fungi could produce a MycoCode that would be fully compatible with the BioCode. In considering microscopic organisms, a newly created MycoCode could also inspire those working on Bacteria, Archaea and other microscopic Eukarya. We mycologists have the need and, in the nomenclatorial committees of the International Mycological AssociationFootnote 2 and the Mycological Section of the International Union of Microbiological Societies, the means to accomplish this task. All that mycologists now lack is an excuse to do nothing.


  1. 1 The English translations are from the 1931 Clarendon Press (Oxford) edition, and were prepared by WB Grove and edited by A H R Bullerand C L Shear.

  2. 2 Including the Nomenclature Committee for Fungi, which it is proposed be elected at International Mycological Congresses rather than at International Botanical Congresses as at present (Hawksworth et al. 2009, Norvell et al. 2010), and the International Commission on the Taxonomy of Fungi (a joint Commission with IUMS).


  • Barron GL (1968) The Genera of Hyphomycetes from Soil. Baltimore: Williams & Wilkins.

    Book  Google Scholar 

  • Berbee ML, Taylor JW (1992) 18S ribosomal RNA gene sequence characters place the human pathogen Sporothrix schenckii in the genus Ophiostoma. Experimental Mycology 16: 87–91.

    Article  CAS  Google Scholar 

  • Briquet J (1912) Règles internationales de al nomenclature botanique adoptées par le Congrès international de botanique de Vienne 1905 dèuxieme édition mise au point d’après les décisions du congrès international de botanique de Bruxelles 1910. Jena: Commission de Redaction du Congres, Fischer Verlag.

    Google Scholar 

  • Bruns TD, Fogel R, White TJ, Palmer JD (1989) Accelerated evolution of a false-truffle from a mushroom ancestor. Nature 339: 140–142.

    Article  CAS  PubMed  Google Scholar 

  • Bruns TD, White TJ, Taylor JW (1991) Fungal molecular systematics. Annual Review of Ecology and Systematics 22: 525–564.

    Article  Google Scholar 

  • Burt A, Carter DA, Koenig GL, White TJ, Taylor JW (1996) Molecular markers reveal cryptic sex in the human pathogen Coccidioides immitis. Proceedings of the National Academy of Sciences, USA 93: 770–773.

    Article  CAS  Google Scholar 

  • Cole GT, Samson RA (1979) Patterns of Development in Conidial Fungi. London: Pitman.

    Google Scholar 

  • Crous PW, Slippers B, Wingfield MJ, Rheeder J, Marasas WFO, Philips AJL, Alves A, Burgess T, Barber P, Groenewald JZ (2006) Phylogenetic lineages in the Botryosphaeriaceae. Studies in Mycology 55: 235–253.

    Article  PubMed  PubMed Central  Google Scholar 

  • Federal Register (2005) Possession, use and transfer of select agents and toxins; Final rule. 70. Federal Register 52 (18 March 2005): 13294–13325 (13297).

    Google Scholar 

  • Fisher MC, Koenig GL, White TJ, Taylor JW (2002) Molecular and phenotypic description of Coccidioides posadasii sp. nov., previously recognized as the non-California population of Coccidioides immitis. Mycologia 94: 73–84.

    Article  CAS  PubMed  Google Scholar 

  • Fuckel L (1870) Symbolae mycologicae. Beitrage zur Kenntnis der rheinischen Pilze. Jahrbücher des Nassauischen Vereins für Naturkunde 23–24: 1–495.

    Google Scholar 

  • Gottschalk M, Blanz PA (1984) Highly conserved 5S ribosomal RNA sequences in four rust fungi and atypical 5S rRNA secondary structure in Microstoma juglandis. Nucleic Acids Research 12: 3951–3958.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Greuter W, Garrity G, Hawksworth DL, Jahn R, Kirk PM, Knapp S, McNeill J, Michel E, Patterson DJ, Pyle R, Tindall B (2011) Draft BioCode (2011): principles and rules regulating the naming of organisms. Taxon 60: 201–212.

    Article  Google Scholar 

  • Guadet J, Julien J, Lafay JF, Brygoo Y (1989) Phylogeny of some Fusarium species, as determined by large-subunit rRNA sequence comparison. Molecular Biology and Evolution 6: 227–242.

    CAS  PubMed  Google Scholar 

  • Gueho E, Kurtzman CP, Peterson SW(1989) Evolutionary affinities of heterobasidiomycetous yeast estimated from 18S and 25S ribosomal RNA seqeunce divergence. Systematic and Applied Microbiology 12: 230–236.

    Article  CAS  Google Scholar 

  • Hawksworth DL (2011) Introducing the Draft BioCode (2011). Taxon 60: 199–200.

    Article  Google Scholar 

  • Hawksworth DL, Cooper JA, Crous PW, Hyde KD, Iturriaga T, Kirk PM, Lumbsch HT, May TW, Minter DW, Misra JK, Norvell L, Redhead SA, Rossman AY, Seifert KA, Stalpers JA, Taylor JW, Wingfield MJ (2010) Proposals to make the pre-publication deposit of key nomenclatural information in a recognized repository a requirement for valid publication of organisms treated as fungi under the Code. Mycotaxon 111: 514–519; Taxon 59: 660–662.

    Google Scholar 

  • Hawksworth DL, Crous PW, Dianese JC, Gryzenhout M, Norvell LL, Seifert KA (2009) Proposals to amend the Code to make it clear that it covers the nomenclature of fungi, and to modify the governance with respect to names of organisms treated as fungi. Taxon 58: 658–659; Mycotaxon 108: 1–4.

    Article  Google Scholar 

  • Hawksworth DL, Crous PW, Redhead SA, Reynolds DR, Samson RA, Seifert KA, Taylor JW, Wngfield MJ, et al. (2011) The Amserdam declaration on fungal nomenclature. IMA Fungus 2: 105–112.

    Article  PubMed  PubMed Central  Google Scholar 

  • Hennig W (1966) Phylogenetic Systematics. Urbana: University of Illinois Press.

    Google Scholar 

  • Hibbett DS, Ohman A, Glotzer D, Nuhn M, Kirk P, Nilsson RH (2011) Progress in molecular and morphological taxon discovery in Fungi and options for formal classification of environmental sequences. Fungal Biology Reviews 25: 38–47.

    Article  Google Scholar 

  • Houbraken J, Frisvad JC, Samson RA (2010) Taxonomy of Penicillium citrinum and related species. Fungal Diversity 44: 117–133.

    Article  Google Scholar 

  • Hughes SJ (1953) Conidiophores, conidia, and classification. Canadian Journal of Botany 31: 577–659.

    Article  Google Scholar 

  • Inderbitzin P, Shoemaker RA, O’Neill NR, Turgeon BG, Berbee ML (2006) Systematics and mating systems of two fungal pathogens of opium poppy: the heterothallic Crivellia papaveracea with a Brachycladium penicillatum asexual state and a homothallic species with a Brachycladium papaveris asexual state. Canadian Journal of Botany 84: 1304–1326.

    Article  CAS  Google Scholar 

  • Jumpponen A, Jones KL (2009) Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperate Quercus macrocarpa phyllosphere. New Phytologist 184: 438–448.

    Article  CAS  Google Scholar 

  • Jumpponen A, Jones KL (2010) Seasonally dynamic fungal communities in the Quercus macrocarpa phyllosphere differ between urban and nonurban environments. New Phytologist 186:496–513.

    Article  CAS  Google Scholar 

  • Kendrick B (ed.)(1979) The Whole Fungus: the sexual-asexual synthesis. 2 vols. Ottawa: National Museums of Canada.

    Google Scholar 

  • Koufopanou V, Burt A, Taylor JW (1997) Concordance of gene genealogies reveals reproductive isolation in the pathogenic fungus Coccidioides immitis. Proceedings of the National Academy of Sciences, USA 94: 5478–5482.

    Article  CAS  Google Scholar 

  • Kurtzman CP, Smiley MJ, Johnson CJ, Wickerham LJ, Fuson GB (1980) Two new and closely related heterothallic species, Pichia amyolphila and Pichia mississippiensis: characterization by hybridization and deoxyribonucleic acid reassociation. International Journal of Systematic Bacteriology 30: 208–216.

    Article  CAS  Google Scholar 

  • Mason EW (1933) Annotated account of Fungi received at the Imperial Mycological Institute, List II (Fasc. 2). Mycological Papers 3: 1–67.

    Google Scholar 

  • Mason EW (1937) Annotated account of Fungi received at the Imperial Mycological Institute, List II, Fasc. 3 gen. part. Mycological Papers 4: 69–99.

    Google Scholar 

  • McNeill J, Barrie FR, Burdet HM, Demoulin V, Hawksworth DL, Marhold K, Nicolson DH, Prado J, Silva PC, Skog JE, Wiersema JH (eds) (2006) International Code of Botanical Nomenclature (Vienna Code) adopted by the Seventeenth Interational Botanical Congress, Vienna, Austria, July 2005. [Regnum Vegetabile No. 146.] Ruggell: A.R.G. Gantner Verlag.

    Google Scholar 

  • Norvell LL, Hawksworth DL, Peterson RH, Redhead SA (2010) IMC9 Edinburgh Nomenclature Sessions. IMA Fungus 1: 143–147; Mycotaxon 113: 503–511.

    Article  PubMed  PubMed Central  Google Scholar 

  • Rabinow P (1996) Making PCR: a story of biotechnology. Chicago: University of Chicago Press.

    Book  Google Scholar 

  • Raper KB, Fennell DI (1965) The Genus Aspergillus. Baltimore: Williams & Wilkins.

    Google Scholar 

  • Reynolds DR, Taylor JW (1991) DNAspecimensand the ‘International code of botanical nomenclature’. Taxon 40: 311–315.

    Article  Google Scholar 

  • Reynolds DR, Taylor JW (1992) Article 59: reinterpretation or revision? Taxon 42: 91–98.

    Article  Google Scholar 

  • Reynolds DR, Taylor JW (eds) (1993) The Fungal Holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. Wallingford: CAB International.

    Google Scholar 

  • Saccardo PA (1882) Sylloge Fungorum omnium hucusque cognitorum. Vol. 1. Pavia: PA Saccardo.

    Book  Google Scholar 

  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich H (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491.

    Article  CAS  PubMed  Google Scholar 

  • Taylor JW (1995) Making the Deuteromycota redundant: a practical integration of mitosporic and meiosporic fungi. Canadian Journal of Botany 73 (Suppl.): s754–s759.

    Article  Google Scholar 

  • Taylor JW, Turner E, Townsend JP, Dettman JR, Jacobson D (2006) Eukaryotic microbes, species recognition and the geographic limits of species: examples from the kingdom Fungi. Philosophical Transactions of the Royal Society B-Biological Sciences 361: 1947–1963.

    Article  PubMed Central  Google Scholar 

  • Tubaki K (1958) Studies on the Japanese Hyphomycetes, 5. Leaf and stem group with a discussion of the classification of Hyphomycetes and their perfect stages. Journal of the Hattori Botanical Laboratory 20: 142–244.

    Google Scholar 

  • Tulasne L-R, Tulasne C (1861) Selecta Fungroum Carpologia. Vol. 1. Paris: Imperial Typographer. [Reprint (1931) Oxford: Clarendon Press.]

    Google Scholar 

  • Vuillemin P (1910a) Les aleuriospores. Bulletin de la Société des Sciences du Nancy 12: 151–175.

    Google Scholar 

  • Vuillemin P (1910b) Les conidiospores. Bulletin de la Société des Sciences du Nancy 11: 129–172.

    Google Scholar 

  • Walker WF, Doolittle WF (1982) Redividing the basidiomycetes on the basis of 5S rRNA sequences. Nature 299: 723–724.

    Article  CAS  PubMed  Google Scholar 

  • Walker WF, Doolittle WF (1983) Systematics of basidiomycetes based on 5S rRNA sequences and other data. Nature 303: 731–732.

    Google Scholar 

  • Weresub LK, Pirozynski KA (1979) Pleomorphism of fungi as treated in the history of mycology and nomenclature. In: The Whole Fungus (B Kendrick, ed.) 1: 17–25. Ottawa: National Museums of Canada.

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor W (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a guide to methods and applications (M Innis et al., eds): 315–322. Academic Press, San Diego.

    Google Scholar 

Download references


Thanks are due to Pedro Crous and Robert Samson of the CBS, Utrecht, who conceived of and hosted the One Fungus = One Name Conference, on 19–20 April 2011 in Amsterdam. JWT also acknowledges support from NSF DEB-0516511

Author information

Authors and Affiliations


Corresponding author

Correspondence to John W. Taylor.

Additional information


Dedication|Dedicated to Don Reynolds, mycological iconoclast, whose sabbatical visit to Berkeley from the Los Angeles County Museum of Natural History more than 20 years ago stimulated thought about One Fungus = One Name.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and permissions

About this article

Cite this article

Taylor, J.W. One Fungus = One Name: DNA and fungal nomenclature twenty years after PCR. IMA Fungus 2, 113–120 (2011).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:

Key words